Development and utilization of a new chemically‐induced soybean library with a high mutation density†
نویسندگان
چکیده
Mutagenized populations have provided important materials for introducing variation and identifying gene function in plants. In this study, an ethyl methanesulfonate (EMS)-induced soybean (Glycine max) population, consisting of 21,600 independent M2 lines, was developed. Over 1,000 M4 (5) families, with diverse abnormal phenotypes for seed composition, seed shape, plant morphology and maturity that are stably expressed across different environments and generations were identified. Phenotypic analysis of the population led to the identification of a yellow pigmentation mutant, gyl, that displayed significantly decreased chlorophyll (Chl) content and abnormal chloroplast development. Sequence analysis showed that gyl is allelic to MinnGold, where a different single nucleotide polymorphism variation in the Mg-chelatase subunit gene (ChlI1a) results in golden yellow leaves. A cleaved amplified polymorphic sequence marker was developed and may be applied to marker-assisted selection for the golden yellow phenotype in soybean breeding. We show that the newly developed soybean EMS mutant population has potential for functional genomics research and genetic improvement in soybean.
منابع مشابه
Identification of Sequence Variation in the Apolipoprotein A2 Gene and Their Relationship with Serum High-Density Lipoprotein Cholesterol Levels
Background: Apolipoprotein A2 (APOA2) is the second major apolipoprotein of the high-density lipoprotein cholesterol (HDL-C). The study aim was to identify APOA2 gene variation in individuals within two extreme tails of HDL-C levels and its relationship with HDL-C level. Methods: This cross-sectional survey was conducted on participants from Tehran Glucose and Lipid Study (TLGS) at Research Ins...
متن کاملImprovement of qualitative and quantitative traits in soybean [Glycine Max (L.) Merrill] through gamma irradiation
Gamma irradiation was used at different doses (80, 160 and 240 Gy) on Glycine Max (L.) Merrill cv. Hill homogenous seeds. A single suitable M2 plant was selected and evaluated at M3 and M4 along with its parent and three other varieties as control in RCBD experiment in Sari Agricultural Sciences and Natural Resources University’s experimental field. M-8...
متن کاملProduction of soybean meal-based feed and its effect on growth performance of western white shrimp (Litopenaeus vannamei) in earthen pond
The effects of two diets, a control diet (commercial feed with 39% crude protein) and an experimental diet (prepared based on 42% soybean meal with 38% crude protein), on growth performance of western white shrimp (Litopenaeus vannamei) in six 0.4-ha-earthen ponds (three replications per treatment) with 25 per m2 density, were investigated. There was no significant difference in final weight (m...
متن کاملHIGH TEMPERATURE TENSILE PROPERTIES OF NEW FE-CR-MN DEVELOPED STEEL
Nowadays, Ni-free austenitic stainless steels are being developed rapidly and high price of nickel is one of the most important motivations for this development. At present research a new FeCrMn steel was designed and produced based on Fe-Cr-Mn-C system. Comparative studies on microstructure and high temperature mechanical properties of new steel and AISI 316 steel were done. The results ...
متن کاملAssessment of seed storage protein composition of six Iranian adopted soybean cultivars [Glycine max (L.) Merrill.]
Seed protein quality is an important topic in the production of soybean. The quality of soybean proteins is limited by anti-nutrient proteins and low levels of essential sulfur amino acids. In this study, protein content and solubility of six cultivars were evaluated and seed storage proteins were analyzed using SDS-PAGE and scanning densitometry. The results showed that seed storage protein ba...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره 59 شماره
صفحات -
تاریخ انتشار 2017